Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hampton Research HR2-243/StockOptions MES, pH 5.2 to 7.1 in 0.1 pH unit increments - 10 ml, tube format
HR2-243/StockOptions MES, pH 5.2 to 7.1 in 0.1 pH unit increments - 10 ml, tube format. Please note items from this supplier are non-returnable.
Research Products International Corp Triton Lysis Buffer Solution, 500 ML
1X buffer used to lyse cells under non-denaturing conditions.
1X Formulation:
NaCI: 150 mM
TritonX-100: 1%
EDTA: 5 mM
Chemscene ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
Medchemexpress LLC Daratumumab PBS 10mg | 10mg
Daratumumab (PBS) (Anti-Human CD38) is the first-in-class human-specific anti-CD38 monoclonal antibody (IgG1) Daratumumab (PBS) has anti-multiple myeloma (MM) effect Daratumumab (PBS) impairs MM cell adhesion which results in an increased sensitivity of MM to proteasome inhibition[1][2]
Apexbio Technology LLC Deacetylase Inhibitor Cocktail (100X in 70% DMSO) 100x1ml
Deacetylase Inhibitor Cocktail is composed of several compounds that act synergistically to maintain the acetylation status of molecules The balance between acetyltransferase and deacetylase activities impacts chromatin compaction transcription factor activity and protein aggregation and the variations of these modifications have garnered considerable interest from researchers Our product Deacetylase Inhibitor Cocktail is a valuable tool for investigating these changes The Deacetylation Inhibition Cocktail includes four active components 40 M Trichostatin A 1 mM EX-527 400 mM nicotinamide and 200 mM sodium butyrate These compounds are dissolved in 70% DMSO each with distinct specific actions Trichostatin A inhibits class I/II HDACs EX-527 selectively inhibits SIRT1 with weaker activity against SIRT2 and SIRT3 Nicotinamide acts as an inhibitor of sirtuins (SIRT1-7 class III HDACs) Sodium butyrate competitively inhibits class I/II HDACs
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3894476 TRIS USP MULTICOM 50KG FLOWMOR
eMolecules Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | 117550-36-4 | MFCD14680132 | 250mg
WuXi AppTec | Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | 250mg | 583186989 | LN01746856 | | 117550-36-4 | MFCD14680132 | 181.191 | C9H11NO3
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Bioworld PBS/Fish Gelatin (10X), 500 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)
Sigma Aldrich Fine Chemicals Biosciences Tris-Glycine Buffer 10x Co1L
TRIS Tris-Glycine Buffer 10x Co1L
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solutin500ML
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Bioworld PBS/Fish Gelatin (10X), 200 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer 5x1L
TRIS Tris-Borate-EDTA buffer 5x1L
Sigma Aldrich Fine Chemicals Biosciences TAPS BioXtra 99.5 titratio100G
TAPS9 TAPS BioXtra 99.5 titratio100G