Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 (titration), 7365-45-9, MFCD00006158
HEPES 99.5 (titration)
Sigma Aldrich Fine Chemicals Biosciences HEPES, 7365-45-9, MFCD00006158, 100g
Empirical Formula (Hill Notation) C8H18N2O4S, ≥99.5% (titration), Molecular Weight 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Hampton Research HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml
HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML
MOPS solution BioPerformance Certified, 1 M, suitable for cell culture | MFCD00006181 | 100ML
MilliporeSigma END COVER PINCH PIPES,3/8X 5/8
NovaSeal™ End cover pinch pipes, 3/8 in. ID x 5/8 in. OD
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 500mL, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Hoefer, Inc MOPS-SDS RUNNING BUFFER 1LX10
Mighty Small precast gels Hoefer Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation.Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution. High Loading Capacity Maximum sample volume is 50 μl Good Repeatability Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability High Resolution Upgraded Bis-Trissystem for clearer, sharper separation bands and higher resolution Highly Effective Maximum 40 min for electrophoresis Ready to use and easy to store No solution preparation or gel casting is required, saving time. Store at 2-8°C Safe and non-toxic No hazardous reagents need to be handled Specifications Plate Size: 10×8 cmWells: 12-well, 15-well Concentration:• Gradient gels: 4-12ĉĉĉĉĉĉĉĉĉĉMOPS-SDS Running Buffer, 1L×10ĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉĉ27-Mar-25 07:45:08:000 PMĉ
Boca Scientific Inc Antibody Stabilizer PBS Base, 50ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline | MFCD00131855 | 100/pk
Phosphate buffered saline | MFCD00131855 | 100/pk
IBA LifeSciences Buffer XT-R - Strep-TactinXT regeneration buffer; 250 ml
Buffer XT-R is a ready-to-use Strep-TactinXT regeneration buffer containing magnesium chloride for competitive displacement of biotin from the modified biotin-binding pocket of Strep-TactinXT after the elution of Strep-tagII or Twin-Strep-tag fusion proteins. Please note that Buffer XT-R is not applicable for Strep-Tactin.
Boston Bioproducts Inc Tris-Acetate Buffer (0.2 M, pH 7.0) - 500ml
The Tris-Acetate Buffer is a buffering agent composed of Tris-base and acetic acid. The combination of these ingredients yields a pH range of 6.0-8.0. This buffer is ideal for requiring a pH slightly above physiological conditions. Often used to support the electrophoresis of nucleic acids on agarose gel and protein electrophoresis employing SDS-PAGE and related blotting techniques.