Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml.
Teknova 10mM Tris-HCl, pH 8.5, 15mL Tube, 25 Tubes per Pack, Sterile. 12 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Bioworld Tris-Triton Buffer, 500 mL
Tris-Triton bufferis the idealbuffer with detergent required to release membrane- or cytoskeleton- bound proteins.Contains: 10mM Tris (pH 7.4), 100mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 10% Glycerol and 0.1% SDSAppearance: Liquid
Teknova Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Boston Bioproducts Inc Bis-Tris Buffer (0.2 M, pH 6.5) - 250ml
The Bis-Tris Buffer is a zwitterionic agent commonly used in biological applications. It maintains a pH within 5.8 to 7.2, ideal for biological processes that require stable environments within this range. It is particularly effective in applications such as protein and nucleic acid purification, biochemical assays, and cell culture. The buffering capacity of Bis-Tris is influenced by its concentration, while its pH can be adjusted based on the ratio of Bis-Tris to its conjugate acid or base.